Purification and Characterization f Two Muconate Cycloisomerase from Aniline - assimilating 1 lyateuria Species ANA - 18
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چکیده
Two muconate cycloisomerases (MC I and MC II, EC 5.5.1.1> were purified to homogeneity from a" anilinegrown R'ateuria sp. ANA-18. MC I and MC II were similar in molecular mass, optimal pH, and pH stability but differny ent in thermostability, and some other enzymatic properties. NH2-terminal amino acid sequences were different between the twe isozymes, indicated that these are encoded by different genes. Different inducible production of MC I and MC II suggested tha"wo catechol branches involyed in the ll-ketoadipate pathway function in R'ateuria sp. ANA-18.
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